Specialister i celledyrkning

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Specialister_Greiner_Billeder



In Vitro has got more than 25 years of specialist experience in sales and guidance of our customers in cell cultivation. We distribute a wide range of consumables products and instruments for cell cultivation, media, serum and reagents for separation and growth of cells and stem cells, as well as many different storage possibilities of cells for medical, pharmaceutical and biotechnological research.

As an exclusive distributor of leading manufacturers in cell cultivation products, our team is continuously taught, trained and introduced to new products by our distributors. Therefore, we are well prepared to guide and assist you with the challenges you may have in your cell culture laboratory. There is no problem that is too small or too big for us. We are driven to surpass our customer’s expectations. So, remember -we are here for you.

One of our biggest supplier in cell cultivations product is Greiner Bio-One, who offers a wide spectrum of cell culture flasks, dishes and plates for different kind of cell types and cell lines.



Surface for Classical Cell applications

Whit the product line CELLSTAR®, which have physically modified surfaces for adherent or suspension cell cultures, Greiner Bio-One offers the ultimate solution for the classical and standard cell culture techniques.  A broad spectrum of formats covers the entire range of applications in cell biology including the propagation of cells, the performance of cell-based assays and elementary imaging procedures. 

Surfaces for Primary and Stem Cells

CELLCOAT® and Advanced TC™ are two platforms available for the cultivation of sensitive cell lines and primary cells. While products in the CELLCOAT® range are coated with extracellular matrix proteins or mimetics such as Collagene Type I, Fibronectin, Laminin, Poly-D-Lysine and Poly-L-Lysine; Advanced TC™ is an animal-free, physicochemical polymer modification proprietary to Greiner Bio-One. Both solutions trace different paths, but have one common goal – the promotion of essential cellular features, such as:

  • Morphology and adherence
  • Proliferation
  • Transgene activity
  • Differentiation status



Advanced TC™ is the first solution for improving assay performance as well as all applications demanding an animal-free substrate supporting sensitive cell lines and primary cells.


CELLCOAT® on the other hand is the ultimate solution for cell culture applications requiring the most native Culture conditions, such as for stem cells.


The CELLSTAR® cell-repellent surface, achieved through an innovative chemical surface modification, reliably prevents cell attachment. As for formation of stem cell aggregates, a key step within many protocols for cultivation and differentiation of stem cells, the cellcell interaction must dominate over the interaction between the cells and the culture surface, cell culture vessels equipped with the Greiner Bio-One cell-repellent surface present an ideal platform.



3D Cell Culture

Vessels equipped with the Greiner Bio-One CELLSTAR® cell-repellent surface support the formation of spheroids, self-assembled spherical clusters used as 3D cell culture models in cancer research and toxicology studies. The CELLSTAR® cell-repellent surface is achieved through an innovative chemical surface modification and reliably prevents cell attachment. Vessels with cell-repellent surface are ideally suited for:

  • Suspension culture of semi-adherent and adherent cell lines
  • Spheroid formation in cancer research and toxicology studies
  • Formation of stem cell aggregates
  • 3D culture in hydrogels



High Quality Solutions for Microscopy

The CELLview™ cell culture dish and slide are high quality platforms for multiplex and live cell imaging experiments. The dish combine the convenience of a standard size 35 mm disposable plastic dish with the premium optical properties of a glass bottom providing researchers with superior high resolution microscopic images of their in vitro cultures. The embedded CELLview™ coverslip guarantees a single-plane flat bottom with a consistent working distance and maximum planarity. The available TC and Advanced TC™ surface modifications make protein coatings dispensable, while improving general cell attachment on the glass substrate.


The CELLview™ slide consists of a transparent slide with a detachable black upper housing that provides 10 wells within individual experiments can be performed. Similar to the CELLview™ dish, the slide has a 175 μm thick cover glass embedded in its bottom to guarantee an even focal plane which is a prerequisite for all high-speed and high-resolution microscopic applications.


SensoPlate™ and SensoPlate™ Plus microplates consist of an optically clear borosilicate glass bottom with a light path of 175 μm and a black polystyrene frame. In addition to fluorescence correlation spectroscopy, microscopic applications such as confocal microscopy are a potential area of application for glass bottom microplates. For mounting the glass bottom plates an adhesive with the lowest possible autofluor escence is used. SensoPlate™ microplates are suited for:

  • Detection of single molecules and fluorescence
  • correlation spectroscopy
  • Sophisticated microscopic applications such as
  • confocal microscopy


Cell Culture Inserts for Complex and Meaningful in vitro Models

When placed in a well plate, ThinCert™ cell culture inserts, with porous PET membranes, form a twocompartment system to readily mimic a variety of in vivo situations, such as:

  • Migration and relocation of cells
  • Paracrine signalling between different cell populations
  • Formation of tight cell-cell junctions and epithelial polarisation
  • Vectorial transport between two lumens
  • Tissue growth and differentiation at the air-liquid-interface


ThinCert™ cell culture inserts combine consistent high quality with a user-friendly format. The PET membrane is firmly attached to the insert housing, but can be easily cut out and subjected to downstream processing, such as immunocytochemistry and sectioning.


Solutions for Automated Cell Culture

Cell culture at the industrial level requires optimized processes that give the best cost-benefit ratios. Specific cell culture labware such as CELLMASTER™ roller bottles meet this requirement. In roller bottles cells grow adherent to the inner lateral surface, while rotation of the device guarantees uniform cell growth as well as alternating supply with nutrients from the culture medium and oxygen from the enclosed air. The enlarged inner lateral surface allows the production of large amounts of cells or cell products in one roller bottle.

The Autoflask™ from Greiner Bio-One is a novel cell culture flask with a microplate footprint. Therefore it can be easily used in conjunction with automated cell culture systems. Its valuable featur es include:

  • Barcodes for flask identification and traceability
  • Filter membrane for optimum gas supply
  • Slit septum for safe liquid transfer
  • Centrifugation pocket for in-flask cell pelletting and harvesting 




Serum and medie for Cell Cultivation

One of our biggest supplier in serum, media and reagents for cell cultivation is Biological Industries. Biological Industries have more than 30 years of experience in cell culture media products designed to support growth and maintenance of a variety cells and cell lines.

Fetal Bovine Serum, also known as Fetal Calf Serum (FCS), is the most widely used growth supplement for cell culture media due to its high content of embryonic growth-promoting factors. Biological Industries’ FBS reagents undergo the most stringent quality control screening and validation standards to ensure high quality and consistent batches with minimal variability. All of BI’s FBS reagents and media meet and exceed approved compliance validation specifications.

 

Serum-free Media

Serum-free and serum-replacement medium formulations have become essential for the future growth of the biotechnology industries. The elimination of animal serum-borne components aims to advance standardization, consistency and to reduce risk of contamination by serum-borne adventitious agents in cell culture processes. Serum-free media and reagents have a wide range of applications, including production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines.

 

Stem cell research

For stem cell research, Biological Industries offers a specific set of xeno-free, animal-free cell culture media, stem cell reagents, and protocols. Successful culture of stem cells requires optimized protocols that incorporate high quality, xeno-free reagents. hESC and hiPSC research is one of the most dynamic fields in modern biology, but cell-based clinical applications are currently limited by xeno-contamination during the in-vitro derivation and propagation phases. Thus, bridging the gap between research models and clinical applications requires the design and implementation of qualified protocols and operating processes. Xeno-free or animal component-free media is an essential element in the development of regenerative stem cell therapies where implantation in humans is the desired outcome.

For Stem Cell Culturing Biological Industries recommend:

NutriStem® V9 XF Medium

  • Defined, serum-free, xeno-free, cGMP
  • Maintains human ES and iPS cell pluripotency at high passage
  • Superior results using Vitronectin
  • Precoating- free option: plating cells without first precoating with vitronectin

 

Stem Cell Differentiation

Whether for basic research, drug discovery or for therapeutic applications, stem cell differentiation requires standardized culture methods to ensure reproducible and reliable results. Biological Industries offers a unique line of MSCgo™ serum-free and xeno-free differentiation media, providing the ability to efficiently differentiate human mesenchymal stem cells from various sources (hMSC-AT, hMSC-BM and hMSC-CT) into adipocytes, chondrocytes and osteoblasts. MSCgo™ Differentiation Media contains all the growth factors and supplements necessary for the directed differentiation of hMSCs.

 

Cryopreservation media

When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation. Biological Industries has developed a family of novel cell cryopreservation formulations which are protein-free, serum-free and animal components-free. Their portfolio of cryopreservation media has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing. Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

Cell Culture Reagents ect.

Biological Industries also offers a wide array of antibiotic, Balanced Salt solutions, reagents, Trypsin and Cell Dissociations Reagents as well as Cytokines, Growth Factors, Chemokines, Hormones, Enzymes, Viral Antigens and many other recombinant proteins used in cell culture.  



Secure sample storage efficient Identification

Our biggest supplier within Storage Solutions and Sample Preservation is Micronic.  Micronic offers a complete line of Sample Storage Tubes. The comprehensive range includes tubes with volumes ranging from 0,5ml to 6,00ml which are suitable for the most common Laboratory Storage applications. The tubes are available non-coded, alphanumeric coded or with a unique 2D Data-Matrix code on the buttom.

All Micronic Storage rack are based on the global recognized ANSI/SLAS standards and provide an easy and automation friendly methods to store your sample storage tube. The racks feature a laser etched 1D rack barcode and a twist-lock to prevent tubes from turning during screw (de)capping. Beside the standard barcoded racks, Micronic also offers the service to supplye laser-etched 1D barcodes in customized formats.  

Micronic offers the most extensive range of tube capping products, including screw caps and push caps. For effective cryogenic storage a screw cap is recommended. For storage from -80CO and above, either a push cap (TPE capcluster) or a screw cap can be used. Alle tubes have an upper screw thread or double o-ring design providing a perfect seal an clouser ensuring maximum sample integrity. (Micronic also offers a wide range of manual and automated capping and decapping equipment.)
 


Efficient sample Identification

To enable fast, accurate and efficient sample identifications, Micronic offers the extensive Tracxer cods reader rang which consists of a single tube scanner and a whole rack scanner.

The Tracxer Code Reader Software is fully compatible with all Micronic rack scanners. The user friendly software is easy to install, gives reliable code readings and can be easily integrated into any laboratory sample database. All features are included as standard in the software at no extra cost together with free upgrades and technical support. 

The ActiveX toolkit included in the Tracxer Code Reader Software enables users to integrate the Tracxer Software easily into their own laboratory information management systems (LIMS). With the ActiveX toolkit, users are able to activate the scanning process of their Tracxer Code Reader with a function inside their sample management database program. 


Tracxer Code Reading Software

The Tracxer Code Reader RS210 is a simple and high-performance scanning unit. With a couple of mouse clicks in the Tracxer Code Reader Software you will be able to scan one full rack (in 12, 24, 48, 96 or 384 SBS format) of 2D Data-Matrix or TraXis coded tubes in less than 5 seconds or a single 96 well format tube in less than 2 seconds. A text file (CSV) is automatically generated containing a list with the tube codes. Due to the inclusion of an ActiveX toolkit in the software, the text file can be interfaced with several types of databases as well as with Excel spreadsheets.


Specialister i Celledyrkning